Chemistry Lab Safety Standard Operating Procedure (SOP)

DMRSTI-Dr. Engineer Mamunur Rashid Science and Technology Institute

Objective: To establish and maintain a safe working environment for all individuals in the chemistry lab and to ensure compliance with safety regulations and practices.

Responsibilities:

1. Lab Supervisor: Responsible for overall lab safety, including training, equipment maintenance, and incident reporting.
2. Lab Technicians: Responsible for enforcing safety rules, providing guidance, and assisting with emergencies.
3. Students: Must follow safety guidelines, report incidents, and participate in safety training.

General Safety Rules:

1. Personal Protective Equipment (PPE):

• Lab coats, safety goggles, and gloves must be worn at all times.
• Long hair should be tied back, and loose clothing and jewelry should be secured.
• Closed-toed shoes with adequate grip are mandatory.

1. Emergency Equipment:

• Familiarize yourself with the location of safety showers, eyewash stations, fire extinguishers, and first-aid kits.
• Know the evacuation routes and assembly points.

1. Chemical Handling:

• Label all chemicals clearly and accurately.
• Never taste or smell chemicals.
• Handle chemicals in a well-ventilated fume hood when required.
• Use appropriate containers for waste disposal.

1. Glassware and Equipment:

• Inspect glassware for cracks or defects before use.
• Report damaged equipment immediately for repair or replacement.

1. Heating and Flames:

• Use Bunsen burners and hot plates with caution.
• Keep flammable materials away from open flames.
• Turn off heating sources when not in use.

1. Spills and Accidents:

• Report all spills, accidents, and injuries to the lab supervisor immediately.
• Use chemical spill kits as needed.
• If clothing catches fire, stop, drop, and roll.

1. Waste Disposal:

• Follow proper disposal procedures for solid and liquid waste.
• Dispose of hazardous waste in designated containers.

1. Equipment Use:

• Use lab equipment only for its intended purpose.
• Clean and return equipment after use.
• Do not modify or repair equipment without authorization.

1. No Eating or Drinking:

• Do not consume food or beverages in the lab.
• Wash hands thoroughly after working in the lab.

Specific Equipment and Procedures:

1. Electronic (Digital) Balance:

• Calibrate the balance before use.
• Handle weights with care to avoid contamination.

1. Distillation Apparatus:

• Follow specific distillation procedures as instructed.

1. pH Meter:

• Calibrate the pH meter regularly.
• Rinse electrodes with deionized water before and after use.

1. Burette and Pipettes:

• Rinse with appropriate solution before use.
• Use a pipette filler for precision and safety.

1. Volumetric and Graduated Flasks:

• Rinse with the intended solution before use.
• Handle with care to avoid breakage.

1. Heating Equipment:

• Maintain a safe distance from hot surfaces.
• Use appropriate clamps and stands for heating.

1. Safety Showers and Eyewash Stations:

• Know the location and operation of these emergency devices.
• Flush eyes or body for at least 15 minutes in case of exposure.

1. Incident Reporting:

• Report all accidents, near misses, and safety concerns to the lab supervisor immediately.

1. Basic Laboratory Equipment:

Laboratory benches and workspaces

Fume hood or ventilation system

Safety shower and eyewash station

Fire extinguishers and first-aid kits

Chemical spill kits

Lab coats, gloves, safety goggles, and other personal protective equipment

1.  Glassware and Labware:

Beakers (various sizes)

Erlenmeyer flasks (various sizes)

Test tubes and test tube racks

Graduated cylinders

Pipettes (volumetric, graduated, and micropipettes)

Burettes

Separatory funnels

Petri dishes

Watch glasses

Glass stirring rods

Thermometers (mercury or digital)

Glass tubing and connectors

Glass slides and coverslips

Mortar and pestle

1. Chemical Storage and Handling:

Chemical storage cabinets

Labeling equipment for chemicals

Chemical spill containment trays

Chemical-resistant gloves

Chemical waste disposal containers

1. Heating and Reaction Equipment:

Bunsen burners or gas burners

Hot plates

Heating mantles

Sand baths

Water baths

Magnetic stirrers and stir bars

Thermocouples and temperature controllers

Analytical Instruments:

Spectrophotometer

pH meters

Conductivity meters

Analytical balances

Centrifuges

Chromatography equipment (HPLC, GC, TLC)

Mass spectrometer (optional, for advanced labs)

1. Safety and Emergency Equipment:

Emergency shower and eyewash station

Fire extinguishers and fire blankets

Emergency exits and evacuation plan

First-aid kit

Chemical spill kits

Safety showers and eyewash stations

1. Additional Supplies:

Laboratory notebooks

Lab markers and labels

Disposable pipette tips and syringes

Weighing boats and paper

Chemical reagents and solutions (various types)

pH buffers and indicators

Lab aprons

Waste disposal containers (for solid and liquid waste)

Desiccators

Vacuum filtration apparatus

Laboratory balances (precision and analytical)

Lab-specific software for data analysis (if needed)

1. Specific List:

Sl. No	Name of Equipment	Quantity
1	Electronic (digital) Balance	02
2	Test Weight ( For Analytical Purpose)	04
3	Distillation Apparatus	01
4	Digital to loading Balance	01
5	PH Meter	04
6	Kipps Apparatus (Complete Set) a) 50 ml ; b) 1000ml	02+02
7	Burette (Borosil-50 ml, 27oC, Readout-0.1 ml	10
8	Pipette: 10 ml; 20 ml; 25 ml	10+05+05
9	Graduated Pipette: 5 ml; 10 ml	05
10	Micro-Pipettes( digital): 10 uliter-100 uliter	02
11	Pipette Controller : 2ml-10 ml
12	Pipette Filler ( Universal type)	05
13	Volumetric Flask: 50;100;200;500 and 1000 ml	5+10+10+5+5
14	Beaker : 50;100;250;500 and 1000 ml	2+10+20+5+5
15	Conical Flask: 100;250;500 and 1000 ml	10+20+5+5
16	Conical Flask with Side Table: 1000 and 500 ml	02+04
17	Weighing Bottle	10
18	Funnel with long tube: 06 cm and 08 cm Dia.	20+10
19	Porcelain Basin: 06 cm and 08 cm Dia.
20	Test Tube: 12.5cm ; 15 cm length	250+250
21	Hard Glass Test Tube : 20 cm length	10+10+5
22	Fusion Tube Standard : 5 cm length	24+10
23	Capillary Tube (Standard)	5
24	Reagent Bottle with Dropper: 250,200, 100 ml	5+10+10
25	Reagent Bottle with Dropper: 250, 100 ml	24+10
26	Flat Bottom Flask: 1000,500, 250ml	5+5+10
27	Round Bottom Flask: 500, 250ml	5+5
28	Blow Pipe (Nickel Plated Brass): 250 ml	12
29	Wolf’s Bottle with Two Necks: 250, 500 ml	10+05
30	Wolf’s Bottle with Three  Necks: 250, 500 ml	05+02
31	Gas Jar with Cover: 250 ; 500 ml	10+05
32	Porcelain Crucible with Lid: 04-05 cm Dia.	10+10
33	Porcelain mortar with Pestle : 06, 08, 12.5 cm Dia.	10+01+05
34	Watch Glass Size: 06 cm; 09 cm Dia.	10+10
35	Electric Oven	10
36	Viscometer digital display	02
37	Melting Point apparatus	01
38	Magnetic Point Stirrer and Hot Plate	01
39	Hand Held Refractometer
40	Conductivity meter
41	Water Purifier (Plastic Body)
42	Chemical Dispenser	02
43	Atomic Model	05
44	Platinum Wire ( Complete Set)	05
45	Blue Glass	10
46	Measuring Cylinder : 5,10,100, 500 and 1000 ml	10+5+2+2+2
47	Bunsen Burner with flame spreader	12
48	Spirit lamp (Standard Size)	12
49	Tripoid Stand ( Circular and triangular)	12
50	Desiccators	02
51	Lie big condenser: 40 , 50 cm	02+01
52	Boiling Bath: 250 ml	06
53	Char coal Block	20
54	Test Tube and Stand	20
55	Stand with Clamp	10
56	Retost with Standard Clamp: 250; 500 ml	05+05
57	Rubber cork	20
58	Magnet (bar)	02
59	Cork Borer set	02
60	Cork presser ( standard size)	02
61	Cork as sorted hand glass	05

 

 

This SOP outlines general and specific safety guidelines for the chemistry lab. Any deviations from these procedures must be approved by the lab supervisor. Regular safety drills and training sessions will be conducted to ensure all lab personnel are familiar with these protocols.

 

Therefore, the Chemistry Lab Safety Standard Operating Procedure (SOP) for DMRSTI-Dr. Engineer Mamunur Rashid Science and Technology Institute has been established with the primary goal of fostering a safe and secure environment for all occupants of our chemistry laboratory.

 

The importance of adhering to these safety guidelines cannot be overstated. They are designed not only to protect the well-being of individuals but also to ensure the integrity and success of experiments and research conducted within the laboratory.

 

By consistently following the safety protocols outlined in this SOP, we commit ourselves to minimizing risks, preventing accidents, and responding effectively to any unexpected incidents that may occur during laboratory activities. Furthermore, these practices align with regulatory requirements and industry standards, positioning our institution as a responsible and conscientious entity in the field of science and technology education.

 

This SOP will be subject to periodic review and updates as necessary to incorporate improvements and changes in safety practices. It is the collective responsibility of all lab personnel, including students, technicians, and instructors, to familiarize themselves with these guidelines and actively participate in maintaining a safe and productive laboratory environment.

 

Through unwavering dedication to safety, we strive to create an environment where scientific exploration can flourish without compromising the well-being of individuals or the integrity of our research. By working together and upholding these safety standards, we can achieve our mission of academic excellence and innovation in the field of chemistry.

 

Approved by Authority

Najmus Sakib Rashid

Rector and Managing Director

Dr. Engineer Mamunur Rashid Science and Technology Institute, Birganj, Dinajpur.

September 16, 2023